GORULE Violations Assigned by WB

Generated by GOA at EMBL-EBI logo - 2026-07-25 08:02:46.156376

Total Lines

339896

WB

907

Valid Lines

884 (97.46%)

Total Problems Detected

23

File Header

!gpa-version: 1.2
!collation date: 2026-07-22
RuleDescriptionCount
✅GORULE:0000001 GAF lines are parsed according to GAF 2.2 specifications 0
✅GORULE:0000002 No 'NOT' annotations to binding ; GO:0005488 or 'protein binding ; GO:0005515' 0
✅GORULE:0000005 IEA, ISS, ISO, ISM, ISA, IBA, RCA annotations are not allowed for direct annotations to protein binding ; GO:0005515 or GO:0005488 binding 0
✅GORULE:0000006 IEP and HEP usage is restricted to terms from the Biological Process ontology, except when assigned by GOC 0
✅GORULE:0000007 IPI should not be used with GO:0003824 catalytic activity or descendants 0
✅GORULE:0000008 No annotations should be made to uninformative high level terms 0
⛔️GORULE:0000011 ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND 15
✅GORULE:0000013 Taxon-appropriate annotation check 0
✅GORULE:0000015 Dual species taxon check 0
✅GORULE:0000016 With/From: IC annotations require a With/From GO ID 0
✅GORULE:0000017 IDA annotations must not have a With/From entry 0
✅GORULE:0000018 IPI annotations require a With/From entry 0
✅GORULE:0000020 Automatic repair of annotations to merged or obsoleted terms 0
✅GORULE:0000022 Check for, and filter, annotations made to retracted publications 0
⛔️GORULE:0000027 Each identifier in GAF is valid 7
⚠️GORULE:0000030 Obsolete GO_REFs are not allowed 1
⛔️GORULE:0000033 Group-specific Reference IDs are not allowed, only public reference IDs (PMID, doi, or GO_REF) 1
✅GORULE:0000039 Protein complexes can not be annotated to GO:0032991 (protein-containing complex) or its descendants 0
✅GORULE:0000042 Qualifier: IKR evidence code requires a NOT qualifier 0
✅GORULE:0000043 Check for valid combination of evidence code and GO_REF 0
✅GORULE:0000046 The 'with' field (GAF column 8) must be the same as the gene product (GAF column 2) when annotating to 'self-binding' terms. 0
✅GORULE:0000050 Annotations to ISS, ISA and ISO should not be self-referential 0
✅GORULE:0000053 'NOT' annotation should not have extension 0
✅GORULE:0000055 References should have only one ID per ID space 0
✅GORULE:0000063 Annotations using ISS/ISA/ISO evidence should refer to a gene product (in the 'with' column) 0
✅GORULE:0000065 Term candidate for obsoletion 0

Messages

✅ GORULE:0000001-GAF lines are parsed according to GAF 2.2 specifications

  • All checks passed - 0 violations

✅ GORULE:0000002-No 'NOT' annotations to binding ; GO:0005488 or 'protein binding ; GO:0005515'

  • All checks passed - 0 violations

✅ GORULE:0000005-IEA, ISS, ISO, ISM, ISA, IBA, RCA annotations are not allowed for direct annotations to protein binding ; GO:0005515 or GO:0005488 binding

  • All checks passed - 0 violations

✅ GORULE:0000006-IEP and HEP usage is restricted to terms from the Biological Process ontology, except when assigned by GOC

  • All checks passed - 0 violations

✅ GORULE:0000007-IPI should not be used with GO:0003824 catalytic activity or descendants

  • All checks passed - 0 violations

✅ GORULE:0000008-No annotations should be made to uninformative high level terms

  • All checks passed - 0 violations

⛔️ GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND

  • Total Violations: 15
  • Line 26 – WB WBGene00011979 enables GO:0003674 GO_REF:0000015 ECO:0000307 20181112 WB model-state=production|contributor=https://orcid.org/0000-0002-3013-9906|noctua-model-id=gomodel:568b0f9600000284
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 1137 – WB WBGene00009783 enables GO:0003674 GO_REF:0000015 ECO:0000307 20180823 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 1177 – WB WBGene00011115 enables GO:0003674 GO_REF:0000015 ECO:0000307 20180828 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 1200 – WB WBGene00020109 enables GO:0003674 GO_REF:0000015 ECO:0000307 20180822 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 1367 – WB WBGene00013765 enables GO:0003674 GO_REF:0000015 ECO:0000307 20180816 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 1852 – WB WBGene00008495 enables GO:0003674 GO_REF:0000015 ECO:0000307 20181210 WB model-state=production|noctua-model-id=gomodel:5b91dbd100001959|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 1853 – WB WBGene00007875 enables GO:0003674 GO_REF:0000015 ECO:0000307 20191015 WB model-state=production|noctua-model-id=gomodel:5b91dbd100001959|contributor=https://orcid.org/0000-0002-1706-4196
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 1862 – WB WBGene00016783 enables GO:0003674 GO_REF:0000015 ECO:0000307 20181211 WB noctua-model-id=gomodel:5b91dbd100001993|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 1867 – WB WBGene00015574 enables GO:0003674 GO_REF:0000015 ECO:0000307 20210512 WB noctua-model-id=gomodel:5b91dbd100001993|model-state=production|contributor=https://orcid.org/0000-0002-1706-4196
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 1934 – WB WBGene00000556 enables GO:0003674 GO_REF:0000015 ECO:0000307 20190107 WB noctua-model-id=gomodel:5b91dbd100002241|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 2880 – WB WBGene00000014 enables GO:0003674 GO_REF:0000015 ECO:0000307 20190814 WB model-state=production|noctua-model-id=gomodel:5d29221b00001060|contributor=https://orcid.org/0000-0002-1706-4196
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 303631 – WB WBGene00001327 enables GO:0003674 GO_REF:0000015 ECO:0000307 20200420 WB noctua-model-id=gomodel:WB_WBGene00001327|model-state=production|contributor=https://orcid.org/0000-0002-1706-4196
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 303633 – WB WBGene00001327 is_active_in GO:0005575 GO_REF:0000015 ECO:0000307 20200513 WB noctua-model-id=gomodel:WB_WBGene00001327|model-state=production|contributor=https://orcid.org/0000-0002-1706-4196
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 303920 – WB WBGene00077700 enables GO:0003674 GO_REF:0000015 ECO:0000307 20120713 WB model-state=production|noctua-model-id=gomodel:WB_WBGene00077700|contributor=https://orcid.org/0000-0002-1706-4196
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND
  • Line 303921 – WB WBGene00077700 is_active_in GO:0005575 GO_REF:0000015 ECO:0000307 20200513 WB model-state=production|noctua-model-id=gomodel:WB_WBGene00077700|contributor=https://orcid.org/0000-0002-1706-4196
    GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND

✅ GORULE:0000013-Taxon-appropriate annotation check

  • All checks passed - 0 violations

✅ GORULE:0000015-Dual species taxon check

  • All checks passed - 0 violations

✅ GORULE:0000016-With/From: IC annotations require a With/From GO ID

  • All checks passed - 0 violations

✅ GORULE:0000017-IDA annotations must not have a With/From entry

  • All checks passed - 0 violations

✅ GORULE:0000018-IPI annotations require a With/From entry

  • All checks passed - 0 violations

✅ GORULE:0000020-Automatic repair of annotations to merged or obsoleted terms

  • All checks passed - 0 violations

✅ GORULE:0000022-Check for, and filter, annotations made to retracted publications

  • All checks passed - 0 violations

⛔️ GORULE:0000027-Each identifier in GAF is valid

  • Total Violations: 7
  • Line 1184 – WB WBGene00005648 enables GO:0004867 GO_REF:0000033 ECO:0000318 Panther:PTN000156122 20180719 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000027-Each identifier in GAF is valid-Prefix not found in db-xrefs: Panther
  • Line 1250 – WB WBGene00015752 enables GO:0003756 GO_REF:0000033 ECO:0000318 Panther:PTN000432607 20180720 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000027-Each identifier in GAF is valid-Prefix not found in db-xrefs: Panther
  • Line 1278 – WB WBGene00015168 enables GO:0003756 GO_REF:0000033 ECO:0000318 Panther:PTN000432607 20180718 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000027-Each identifier in GAF is valid-Prefix not found in db-xrefs: Panther
  • Line 1298 – WB WBGene00015057 enables GO:0004854 GO_REF:0000033 ECO:0000318 Panther:PTN000225632 20180709 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000027-Each identifier in GAF is valid-Prefix not found in db-xrefs: Panther
  • Line 1348 – WB WBGene00009178 enables GO:0003980 GO_REF:0000033 ECO:0000318 Panther:PTN000132051 20180710 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000027-Each identifier in GAF is valid-Prefix not found in db-xrefs: Panther
  • Line 1459 – WB WBGene00000503 enables GO:0004568 GO_REF:0000033 ECO:0000318 Panther:PTN000126434 20180718 WB noctua-model-id=gomodel:5b318d0900000658|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000027-Each identifier in GAF is valid-Prefix not found in db-xrefs: Panther
  • Line 33479 – UniProtKB P05198 is_active_in GO:0005829 GO_REF:0000024 ECO:0000250 PomBase:SPAC3G9.09c 20251003 WB contributor=https://orcid.org/0000-0001-7299-6685|noctua-model-id=gomodel:6606056e00000903|model-state=production|contributor=https://orcid.org/0000-0002-1706-4196
    GORULE:0000027-Each identifier in GAF is valid-Bad id syntax for PomBase:SPAC3G9.09c

⚠️ GORULE:0000030-Obsolete GO_REFs are not allowed

  • Total Violations: 1
  • Line 1372 – WB WBGene00021868 enables GO:0140523 GO_REF:0000038 ECO:0000501 UniProtKB-KW:KW-0547 20180824 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000030-Obsolete GO_REFs are not allowed-GO_REF:0000038 reference is not allowed

⛔️ GORULE:0000033-Group-specific Reference IDs are not allowed, only public reference IDs (PMID, doi, or GO_REF)

  • Total Violations: 1
  • Line 1372 – WB WBGene00021868 enables GO:0140523 GO_REF:0000038 ECO:0000501 UniProtKB-KW:KW-0547 20180824 WB noctua-model-id=gomodel:5b318d0900000194|model-state=production|contributor=https://orcid.org/0000-0002-3013-9906
    GORULE:0000033-Group-specific Reference IDs are not allowed, only public reference IDs (PMID, doi, or GO_REF)-GO_REF:0000038 reference is not allowed

✅ GORULE:0000039-Protein complexes can not be annotated to GO:0032991 (protein-containing complex) or its descendants

  • All checks passed - 0 violations

✅ GORULE:0000042-Qualifier: IKR evidence code requires a NOT qualifier

  • All checks passed - 0 violations

✅ GORULE:0000043-Check for valid combination of evidence code and GO_REF

  • All checks passed - 0 violations

✅ GORULE:0000046-The 'with' field (GAF column 8) must be the same as the gene product (GAF column 2) when annotating to 'self-binding' terms.

  • All checks passed - 0 violations

✅ GORULE:0000050-Annotations to ISS, ISA and ISO should not be self-referential

  • All checks passed - 0 violations

✅ GORULE:0000053-'NOT' annotation should not have extension

  • All checks passed - 0 violations

✅ GORULE:0000055-References should have only one ID per ID space

  • All checks passed - 0 violations

✅ GORULE:0000063-Annotations using ISS/ISA/ISO evidence should refer to a gene product (in the 'with' column)

  • All checks passed - 0 violations

✅ GORULE:0000065-Term candidate for obsoletion

  • All checks passed - 0 violations