GORULE Violations Assigned by UOS_MCB

_{Generated by GOA at

- 2026-07-25 08:02:47.801245}

Total Lines

339896

UOS_MCB

401

Valid Lines

398 (99.25%)

Total Problems Detected

File Header

!gpa-version: 1.2

!collation date: 2026-07-22

Rule	Description	Count
✅GORULE:0000001	GAF lines are parsed according to GAF 2.2 specifications	0
✅GORULE:0000002	No 'NOT' annotations to binding ; GO:0005488 or 'protein binding ; GO:0005515'	0
✅GORULE:0000005	IEA, ISS, ISO, ISM, ISA, IBA, RCA annotations are not allowed for direct annotations to protein binding ; GO:0005515 or GO:0005488 binding	0
✅GORULE:0000006	IEP and HEP usage is restricted to terms from the Biological Process ontology, except when assigned by GOC	0
✅GORULE:0000007	IPI should not be used with GO:0003824 catalytic activity or descendants	0
✅GORULE:0000008	No annotations should be made to uninformative high level terms	0
✅GORULE:0000011	ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND	0
✅GORULE:0000013	Taxon-appropriate annotation check	0
✅GORULE:0000015	Dual species taxon check	0
✅GORULE:0000016	With/From: IC annotations require a With/From GO ID	0
✅GORULE:0000017	IDA annotations must not have a With/From entry	0
✅GORULE:0000018	IPI annotations require a With/From entry	0
✅GORULE:0000020	Automatic repair of annotations to merged or obsoleted terms	0
✅GORULE:0000022	Check for, and filter, annotations made to retracted publications	0
⛔️GORULE:0000027	Each identifier in GAF is valid	3
✅GORULE:0000030	Obsolete GO_REFs are not allowed	0
✅GORULE:0000033	Group-specific Reference IDs are not allowed, only public reference IDs (PMID, doi, or GO_REF)	0
✅GORULE:0000039	Protein complexes can not be annotated to GO:0032991 (protein-containing complex) or its descendants	0
✅GORULE:0000042	Qualifier: IKR evidence code requires a NOT qualifier	0
✅GORULE:0000043	Check for valid combination of evidence code and GO_REF	0
✅GORULE:0000046	The 'with' field (GAF column 8) must be the same as the gene product (GAF column 2) when annotating to 'self-binding' terms.	0
✅GORULE:0000050	Annotations to ISS, ISA and ISO should not be self-referential	0
✅GORULE:0000053	'NOT' annotation should not have extension	0
✅GORULE:0000055	References should have only one ID per ID space	0
✅GORULE:0000063	Annotations using ISS/ISA/ISO evidence should refer to a gene product (in the 'with' column)	0
✅GORULE:0000065	Term candidate for obsoletion	0

Messages

✅ GORULE:0000001-GAF lines are parsed according to GAF 2.2 specifications

All checks passed - 0 violations

✅ GORULE:0000002-No 'NOT' annotations to binding ; GO:0005488 or 'protein binding ; GO:0005515'

All checks passed - 0 violations

✅ GORULE:0000005-IEA, ISS, ISO, ISM, ISA, IBA, RCA annotations are not allowed for direct annotations to protein binding ; GO:0005515 or GO:0005488 binding

All checks passed - 0 violations

✅ GORULE:0000006-IEP and HEP usage is restricted to terms from the Biological Process ontology, except when assigned by GOC

All checks passed - 0 violations

✅ GORULE:0000007-IPI should not be used with GO:0003824 catalytic activity or descendants

All checks passed - 0 violations

✅ GORULE:0000008-No annotations should be made to uninformative high level terms

All checks passed - 0 violations

✅ GORULE:0000011-ND evidence code should be to root nodes only, and no terms other than root nodes can have the evidence code ND

All checks passed - 0 violations

✅ GORULE:0000013-Taxon-appropriate annotation check

All checks passed - 0 violations

✅ GORULE:0000015-Dual species taxon check

All checks passed - 0 violations

✅ GORULE:0000016-With/From: IC annotations require a With/From GO ID

All checks passed - 0 violations

✅ GORULE:0000017-IDA annotations must not have a With/From entry

All checks passed - 0 violations

✅ GORULE:0000018-IPI annotations require a With/From entry

All checks passed - 0 violations

✅ GORULE:0000020-Automatic repair of annotations to merged or obsoleted terms

All checks passed - 0 violations

✅ GORULE:0000022-Check for, and filter, annotations made to retracted publications

All checks passed - 0 violations

⛔️ GORULE:0000027-Each identifier in GAF is valid

Total Violations: 3

Line 51073 – UniProtKB Q9BZF3 is_active_in GO:0140268 GO_REF:0000024 ECO:0000250 MGI:2139014 20251027 UOS_MCB part_of(UBERON:0000955) noctua-model-id=gomodel:684b6c8100004263|contributor=https://orcid.org/0009-0002-7487-1936|model-state=production|contributor=https://orcid.org/0000-0001-8769-177X
GORULE:0000027-Each identifier in GAF is valid-Bad id syntax for MGI:2139014
Line 51074 – UniProtKB Q9BZF3 involved_in GO:0120010 GO_REF:0000024 ECO:0000250 MGI:2139014 20251027 UOS_MCB noctua-model-id=gomodel:684b6c8100004263|contributor=https://orcid.org/0009-0002-7487-1936|model-state=production|contributor=https://orcid.org/0000-0001-8769-177X
GORULE:0000027-Each identifier in GAF is valid-Bad id syntax for MGI:2139014
Line 51076 – UniProtKB Q9BZF3 enables GO:0160270 GO_REF:0000024 ECO:0000250 MGI:2139014 20251027 UOS_MCB part_of(GO:0120010),occurs_in(GO:0140268),occurs_in(UBERON:0000955) noctua-model-id=gomodel:684b6c8100004263|contributor=https://orcid.org/0009-0002-7487-1936|model-state=production|contributor=https://orcid.org/0000-0001-8769-177X
GORULE:0000027-Each identifier in GAF is valid-Bad id syntax for MGI:2139014