{"id":"gomodel:SYNGO_5386","individuals":[{"id":"gomodel:SYNGO_538608bc6f49-acd8-47c7-b079-3edd097479d0","type":[{"type":"class","id":"ECO:0006042","label":"cell fractionation evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"source","value":"PMID:33376223"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"}]},{"id":"gomodel:SYNGO_53862c695c2b-60c4-4385-84a4-1bcb98b83a67","type":[{"type":"class","id":"GO:0003674","label":"molecular_function"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"}]},{"id":"gomodel:SYNGO_538630873ac5-dee1-4fd7-aa2f-021f1233cf88","type":[{"type":"class","id":"GO:0008021","label":"synaptic vesicle"}],"root-type":[{"type":"class","id":"UBERON:0001062","label":"anatomical entity"},{"type":"class","id":"GO:0110165","label":"cellular anatomical structure"},{"type":"class","id":"GO:0005575","label":"cellular_component"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"}]},{"id":"gomodel:SYNGO_53865d7e7dec-ecac-4a24-9cbb-cd151dd1dec5","type":[{"type":"class","id":"ECO:0001230","label":"mass spectrometry evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"source","value":"PMID:33376223"}]},{"id":"gomodel:SYNGO_538668855ff1-2637-4d3b-a6c4-42dd3bc5ad72","type":[{"type":"class","id":"RGD:1311115","label":"Ap3s1 Rnor"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"}]},{"id":"gomodel:SYNGO_5386983f37b6-2b16-43e9-806f-f1dccbd76a18","type":[{"type":"class","id":"ECO:0006042","label":"cell fractionation evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:33376223"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"}]},{"id":"gomodel:SYNGO_5386f74f3cc8-4d92-45a5-834c-637421a9efe9","type":[{"type":"class","id":"ECO:0001230","label":"mass spectrometry evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"source","value":"PMID:33376223"}]}],"facts":[{"subject":"gomodel:SYNGO_53862c695c2b-60c4-4385-84a4-1bcb98b83a67","property":"BFO:0000066","property-label":"BFO:0000066","object":"gomodel:SYNGO_538630873ac5-dee1-4fd7-aa2f-021f1233cf88","annotations":[{"key":"evidence","value":"gomodel:SYNGO_538608bc6f49-acd8-47c7-b079-3edd097479d0","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_5386f74f3cc8-4d92-45a5-834c-637421a9efe9","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"subject":"gomodel:SYNGO_53862c695c2b-60c4-4385-84a4-1bcb98b83a67","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_538668855ff1-2637-4d3b-a6c4-42dd3bc5ad72","annotations":[{"key":"evidence","value":"gomodel:SYNGO_53865d7e7dec-ecac-4a24-9cbb-cd151dd1dec5","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_5386983f37b6-2b16-43e9-806f-f1dccbd76a18","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"date","value":"2023-03-15"},{"key":"title","value":"SYNGO_CC_5386"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: AP3S1 (AP3-σ3A) was detected in SV biochemical fractions, but not enriched as compared to the P2 fraction.\n\nAP3S1 (AP3-σ3A) and AP3S2 (AP3-σ3A) were also detected in SV biochemical fractions prepared from rat brain that were subjected to sucrose gradients and a series of western blots (PMID:9590176, Fig.4).\n\nThis protein is part of the ubiquitous AP3 complex (PMID:9118953, PMID:9151686).. Experimental description: M\u0026M: \"Brain Synaptosomes and SV Purifications. Synaptosomes (P2\u0027) and SVs were purified from whole brain of 4- to 6-wk-old Sprague-Dawley rats following the same protocols used in ref. 2 and previously described in ref. 34 for SV, and in ref. 4 for P2\u0027.\""},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_10116"}]}