{"id":"gomodel:SYNGO_533","individuals":[{"id":"gomodel:SYNGO_53305dbd05e-9366-4515-915d-6b5c514e10bd","type":[{"type":"class","id":"ECO:0000303","label":"author statement without traceable support used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"source","value":"PMID:25615535"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_533635b2ab3-9d8e-4089-92f8-44556ec26d65","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"source","value":"PMID:25615535"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_53387b1325b-d9e0-44fe-b469-da62896f3d78","type":[{"type":"class","id":"ECO:0006013","label":"patch-clamp recording evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:25615535"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_533cc4df3d8-d42a-45dc-828a-73ec8c1acace","type":[{"type":"class","id":"RGD:3632","label":"Scn2a Rnor"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_533db43f02a-e680-4704-a603-34705e10931f","type":[{"type":"class","id":"GO:0099508","label":"voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_533feaf9ef7-a55f-43b6-b60c-8f3b6cc270d0","type":[{"type":"class","id":"ECO:0006063","label":"over expression analysis evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"source","value":"PMID:25615535"}]}],"facts":[{"subject":"gomodel:SYNGO_533db43f02a-e680-4704-a603-34705e10931f","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_533cc4df3d8-d42a-45dc-828a-73ec8c1acace","annotations":[{"key":"evidence","value":"gomodel:SYNGO_53305dbd05e-9366-4515-915d-6b5c514e10bd","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_533635b2ab3-9d8e-4089-92f8-44556ec26d65","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_53387b1325b-d9e0-44fe-b469-da62896f3d78","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_533feaf9ef7-a55f-43b6-b60c-8f3b6cc270d0","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2018-06-29"},{"key":"title","value":"SYNGO_MF_533"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: This paper shows that Nav1.2 is a sodium channel. Rat Nav1.2 channels are expressed in HEK cells and characterised using whole cell voltage clamp.\n\nrule: NAS_MFCC-BP\nrationale:\nProtein has voltage-gated ion channel activity and is localized in teh presynaptic membrane (show with high-resolution technique). The inference to term \u0027voltage-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099508)\u0027 is trivial and non-controversial.\nPinf_CC:\n[Q68V27] SyNGO ID 531 (PMID:22528969, EM). Experimental description: p.833: HEK-293 cells stably expressing rat Nav1.2 (HEK-Nav1.2 cells, gift from Dr. David Ornitz, Washington University in St. Louis) were maintained in medium composed of equal volumes of DMEM and F12 (Invitrogen, Carlsbad, CA) supplemented with 0.05% glucose, 0.5 mM pyruvate, 10% fetal bovine serum, 100 U/ml penicillin, 100 μg/mL streptomycin, and 500 μg/ml G418 (Invitrogen) for the selection of Nav1.2 stably transfected cells, and incubated at 37 °C with 5% CO2, as previously described [29]. -- Highlighted 10 May 2017"},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_10116"}]}