{"id":"gomodel:SYNGO_4950","individuals":[{"id":"gomodel:SYNGO_495005bffdfa-0bb6-4f4b-99ac-8625031cf8ae","type":[{"type":"class","id":"GO:0042734","label":"presynaptic membrane"}],"root-type":[{"type":"class","id":"UBERON:0001062","label":"anatomical entity"},{"type":"class","id":"GO:0110165","label":"cellular anatomical structure"},{"type":"class","id":"GO:0005575","label":"cellular_component"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_49502946c5c3-75c4-48b2-9741-a8602d219028","type":[{"type":"class","id":"ECO:0006003","label":"electron microscopy evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:33913808"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_49505a6bf3b4-aa3b-4351-a800-3ddd30867804","type":[{"type":"class","id":"MGI:MGI:2444667","label":"Kctd12b Mmus"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical 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assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"source","value":"PMID:33913808"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_4950e5be689e-8d8a-47c5-937a-0785c94387d3","type":[{"type":"class","id":"ECO:0007719","label":"immunodetection assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:33913808"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_4950fa225291-4da4-453c-8eff-f6548da62f5f","type":[{"type":"class","id":"ECO:0007719","label":"immunodetection assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"source","value":"PMID:33913808"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"facts":[{"subject":"gomodel:SYNGO_49507d4eb9e4-d588-4546-a162-07fe5e566640","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_49505a6bf3b4-aa3b-4351-a800-3ddd30867804","annotations":[{"key":"evidence","value":"gomodel:SYNGO_49502946c5c3-75c4-48b2-9741-a8602d219028","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_4950fa225291-4da4-453c-8eff-f6548da62f5f","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"subject":"gomodel:SYNGO_49507d4eb9e4-d588-4546-a162-07fe5e566640","property":"BFO:0000066","property-label":"BFO:0000066","object":"gomodel:SYNGO_495005bffdfa-0bb6-4f4b-99ac-8625031cf8ae","annotations":[{"key":"evidence","value":"gomodel:SYNGO_4950d399d7a8-7618-4d69-b560-3656da2ad5db","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_4950e5be689e-8d8a-47c5-937a-0785c94387d3","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2023-01-30"},{"key":"title","value":"SYNGO_CC_4950"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: - K+-channel tetramerization domain-containing proteins (KCTDs) are auxiliary GABAB receptor (GBR) subunits.\n- Experiments were performed at the synaptic connection from medial habenula (MHb) to interpeduncular nucleus (IPN).\n- pre-embedding immunogold labeling, imaged with TEM. \"In MHb terminals in the rostral IPN, Cav2.3 and KCTD12b showed similar localization patterns, with peak particle densities in the active zone and a gradual decrease in density with increased distance from the active zone (Figure 3A,E).\"\n\n- Figure 2C: immunolabeling of WT and KCTD12b KO mice shows antibody specificity. Experimental description: Wild-type C57BL/6J mice(Jax, Bar Harbor, ME; #000664) purchased from Jackson Laboratory.\nPotassium channel tetramerisation domain-containing 12b\nAntibody: anti-KCTD12b (polyclonal, raised against a synthetic peptide comprised of the N-terminal amino acids 1-16 of KCTD12b) (1 ug/ul, 1 O/N, Bettler lab, Univ. Basel)"},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_10090"}]}