{"id":"gomodel:SYNGO_4913","individuals":[{"id":"gomodel:SYNGO_491308c4a2c6-31be-446f-90e5-8e103f2ca067","type":[{"type":"class","id":"GO:0003674","label":"molecular_function"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-5615-1014"}]},{"id":"gomodel:SYNGO_4913176ad3cc-c449-4ebe-9e58-92146cc76e43","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-5615-1014"},{"key":"date","value":"2024-03-06"},{"key":"source","value":"PMID:14627637"}]},{"id":"gomodel:SYNGO_49137863b03f-d783-4971-9a24-7573ad4ade35","type":[{"type":"class","id":"ECO:0006063","label":"over expression analysis evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:14627637"},{"key":"contributor","value":"https://orcid.org/0000-0002-5615-1014"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_4913ae134438-bc36-440a-b485-411ad869069f","type":[{"type":"class","id":"GO:0099161","label":"regulation of presynaptic dense core granule exocytosis"}],"root-type":[{"type":"class","id":"GO:0008150","label":"biological_process"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-5615-1014"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"}]},{"id":"gomodel:SYNGO_4913b63ac0d5-c53e-44b2-be9b-9ff03c8dcac2","type":[{"type":"class","id":"ECO:0006063","label":"over expression analysis evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-5615-1014"},{"key":"source","value":"PMID:14627637"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_4913b7b8fbd2-9db2-48e2-8a17-9120126656b4","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:14627637"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-5615-1014"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_4913cd20b5b2-3038-492c-bbf1-ff1dfe81588e","type":[{"type":"class","id":"RGD:68369","label":"Rab11b Rnor"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-5615-1014"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"facts":[{"subject":"gomodel:SYNGO_491308c4a2c6-31be-446f-90e5-8e103f2ca067","property":"BFO:0000050","property-label":"BFO:0000050","object":"gomodel:SYNGO_4913ae134438-bc36-440a-b485-411ad869069f","annotations":[{"key":"evidence","value":"gomodel:SYNGO_4913176ad3cc-c449-4ebe-9e58-92146cc76e43","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_49137863b03f-d783-4971-9a24-7573ad4ade35","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-5615-1014"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"subject":"gomodel:SYNGO_491308c4a2c6-31be-446f-90e5-8e103f2ca067","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_4913cd20b5b2-3038-492c-bbf1-ff1dfe81588e","annotations":[{"key":"evidence","value":"gomodel:SYNGO_4913b63ac0d5-c53e-44b2-be9b-9ff03c8dcac2","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_4913b7b8fbd2-9db2-48e2-8a17-9120126656b4","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-5615-1014"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0002-5615-1014"},{"key":"date","value":"2023-01-31"},{"key":"title","value":"SYNGO_BP_4913"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: Fig 1: Overexpression of Rab11b inhibits high K+ induced release of human growth hormone (hGH) from dense core vesicles in PC12 cells.\nFig 3: Inhibition of hGH release in PC12 cells is independent of GTP/GDP binding and prenylation status of Rab11b.. Experimental description: Source of Rab11B cDNA is unclear. Species derived from Results: Therefore, we prepared synaptosomes (P2) from rat brain, isolated crude synaptic vesicles (LP2) by differential centrifugation from these synaptosomes, and further purified the vesicles by controlled pore-glass chromatography. The chromatography produced two major fractions: peak 1, which contains myelin and other small membrane fragments, and peak 2, which contains pure synaptic vesicles (Nagy et al., 1976).\n\nAntibodies: Glutathione S-transferase (GST)-fused recombinant Rab11b was expressed in bacteria, purified on a glutathione agarose column, and digested with thrombin to remove GST part. Purified recombinant protein was then used as an antigen to produce polyclonal rabbit antibodies against Rab11b (T3746). Antibodies were immunopurified from the serum using standard procedures. Monoclonal antibody against Rab11 was from Transduction Laboratories (San Diego, CA)."},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_10116"}]}