{"id":"gomodel:SYNGO_4757","individuals":[{"id":"gomodel:SYNGO_475709ffe5df-90ea-404a-a03c-ad30883d2ca2","type":[{"type":"class","id":"GO:0003674","label":"molecular_function"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"}]},{"id":"gomodel:SYNGO_47571eec33bf-28e3-455e-8b01-e9d914b7762c","type":[{"type":"class","id":"RGD:1586888","label":"Ap3s2 Rnor"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"}]},{"id":"gomodel:SYNGO_47572f98f513-db7e-423c-b57e-b04ecfa49818","type":[{"type":"class","id":"ECO:0001230","label":"mass spectrometry evidence used in manual 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structure"},{"type":"class","id":"GO:0005575","label":"cellular_component"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_47579b455f3e-7111-45b4-8b9d-d31081fe49c7","type":[{"type":"class","id":"ECO:0001230","label":"mass spectrometry evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"source","value":"PMID:33376223"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"}]},{"id":"gomodel:SYNGO_47579dc4c596-8ff2-4249-b7c8-fe169d800728","type":[{"type":"class","id":"ECO:0006042","label":"cell fractionation evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"source","value":"PMID:33376223"}]}],"facts":[{"subject":"gomodel:SYNGO_475709ffe5df-90ea-404a-a03c-ad30883d2ca2","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_47571eec33bf-28e3-455e-8b01-e9d914b7762c","annotations":[{"key":"evidence","value":"gomodel:SYNGO_47573e41e143-f656-4402-828e-1368979f94de","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_47579b455f3e-7111-45b4-8b9d-d31081fe49c7","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"subject":"gomodel:SYNGO_475709ffe5df-90ea-404a-a03c-ad30883d2ca2","property":"BFO:0000066","property-label":"BFO:0000066","object":"gomodel:SYNGO_47573e7c0b9b-d974-4ab6-8eff-03bb1ae2c20f","annotations":[{"key":"evidence","value":"gomodel:SYNGO_47572f98f513-db7e-423c-b57e-b04ecfa49818","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_47579dc4c596-8ff2-4249-b7c8-fe169d800728","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0002-4973-5732"},{"key":"date","value":"2023-03-15"},{"key":"title","value":"SYNGO_CC_4757"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: AP3S2 (AP3-σ3B) was detected in SV biochemical fractions, but not enriched as compared to the P2 fraction.\n\nThis protein was also detected in SV biochemical fractions in Takamori et al. 2006 (PMID: 17110340).\n\nAP3S1 (AP3-σ3A) and AP3S2 (AP3-σ3A) were also detected in SV biochemical fractions prepared from rat brain that were subjected to sucrose gradients and a series of western blots (PMID:9590176, Fig.4).\n\nThis protein is part of the ubiquitous AP3 complex (PMID:9118953, PMID:9151686).. Experimental description: M\u0026M: \"Brain Synaptosomes and SV Purifications. Synaptosomes (P2\u0027) and SVs were purified from whole brain of 4- to 6-wk-old Sprague-Dawley rats following the same protocols used in ref. 2 and previously described in ref. 34 for SV, and in ref. 4 for P2\u0027.\""},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_10116"}]}