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This annotation is based on inference over multiple papers, as localization of AP2A2 to synapses and implication of the AP2 complex in synaptic endocytosis, for papers that we reviewed, use antibodies that are a-specific for AP2A1 and AP2A2.\n\nHowever, additional mass-spectrometric evidence points to AP2A2 in subcellular enrichments of synaptic fractions for presynapse, postsynapse and synaptic vesicles (where enrichment is observed as compared to P2 fraction).\n\nIn figure 2, the authors prepare synaptosomes (nerve terminals) that they treat with GTPgammaS to trap endocytic vesicles. They then use immunogold with an antibody against AP-2 alpha adaptin to demonstrate the protein at endocytic sites. The antibody used, anti-alpha Adaptin antibody [AP6] (also available as Abcam #2730 / ab2730), targets both AP2A1 and AP2A2.. Experimental description: From the methods, \"Synaptosomal fractions (P2) were prepared from rat brain homogenates as described (Huttner et al., 1983)\"."},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_10116"}]}