{"id":"gomodel:SYNGO_4584","individuals":[{"id":"gomodel:SYNGO_458404249192-f316-4e7c-baf5-961b3ee256a5","type":[{"type":"class","id":"ECO:0006003","label":"electron microscopy evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"source","value":"PMID:33913808"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"}]},{"id":"gomodel:SYNGO_458412235778-7252-484a-989b-ca64187abfa1","type":[{"type":"class","id":"MGI:MGI:2444667","label":"Kctd12b Mmus"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical 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assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"date","value":"2024-03-06"},{"key":"source","value":"PMID:33913808"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_4584f5dd40a4-8934-44ce-96f7-807de399e249","type":[{"type":"class","id":"ECO:0006003","label":"electron microscopy evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"source","value":"PMID:33913808"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"}]}],"facts":[{"subject":"gomodel:SYNGO_458428460f6b-ae6e-4086-b288-bd50e0f9f14e","property":"BFO:0000066","property-label":"BFO:0000066","object":"gomodel:SYNGO_45849bc963ca-a3db-4aef-83ab-5c9449361d6b","annotations":[{"key":"evidence","value":"gomodel:SYNGO_458404249192-f316-4e7c-baf5-961b3ee256a5","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_4584eaea00e0-5691-4020-8205-0f656cc0afb7","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"subject":"gomodel:SYNGO_458428460f6b-ae6e-4086-b288-bd50e0f9f14e","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_458412235778-7252-484a-989b-ca64187abfa1","annotations":[{"key":"evidence","value":"gomodel:SYNGO_4584aa6a2295-398c-48df-92e9-0f881976a6f0","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_4584f5dd40a4-8934-44ce-96f7-807de399e249","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2023-01-30"},{"key":"title","value":"SYNGO_CC_4584"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: - K+-channel tetramerization domain-containing proteins (KCTDs) are auxiliary GABAB receptor (GBR) subunits.\n- Experiments were performed at the synaptic connection from medial habenula (MHb) to interpeduncular nucleus (IPN).\n- pre-embedding immunogold labeling, imaged with TEM. \"In MHb terminals in the rostral IPN, Cav2.3 and KCTD12b showed similar localization patterns, with peak particle densities in the active zone and a gradual decrease in density with increased distance from the active zone (Figure 3A,E).\"\n\n- Figure 2C: immunolabeling of WT and KCTD12b KO mice shows antibody specificity. Experimental description: Wild-type C57BL/6J mice(Jax, Bar Harbor, ME; #000664) purchased from Jackson Laboratory.\nPotassium channel tetramerisation domain-containing 12b\nAntibody: anti-KCTD12b (polyclonal, raised against a synthetic peptide comprised of the N-terminal amino acids 1-16 of KCTD12b) (1 ug/ul, 1 O/N, Bettler lab, Univ. Basel)"},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_10090"}]}