{"id":"gomodel:SYNGO_4537","individuals":[{"id":"gomodel:SYNGO_45372e563eda-078e-4dbd-b870-bb0a77bc1cb1","type":[{"type":"class","id":"GO:0099626","label":"voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0003-2916-7616"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_45373ce0b8e4-ccb3-4d6c-99ba-b67296323ca3","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0003-2916-7616"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"source","value":"PMID:17068255"}]},{"id":"gomodel:SYNGO_45376c42abfb-7e9e-45aa-ba08-ea4a3fe84942","type":[{"type":"class","id":"ECO:0006013","label":"patch-clamp recording evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:17068255"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0003-2916-7616"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_4537a88b4e2b-ebe1-4a22-8b07-5c7646b2f824","type":[{"type":"class","id":"UniProtKB:P27884","label":"CACNA1A NCBITaxon:9986"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"},{"type":"class","id":"CHEBI:36080","label":"protein"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0003-2916-7616"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_4537f9ebaa42-5fb8-44ce-b662-11a7cd0dbb24","type":[{"type":"class","id":"ECO:0006054","label":"pharmacological assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"source","value":"PMID:17068255"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0003-2916-7616"}]}],"facts":[{"subject":"gomodel:SYNGO_45372e563eda-078e-4dbd-b870-bb0a77bc1cb1","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_4537a88b4e2b-ebe1-4a22-8b07-5c7646b2f824","annotations":[{"key":"evidence","value":"gomodel:SYNGO_45373ce0b8e4-ccb3-4d6c-99ba-b67296323ca3","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_45376c42abfb-7e9e-45aa-ba08-ea4a3fe84942","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_4537f9ebaa42-5fb8-44ce-b662-11a7cd0dbb24","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0003-2916-7616"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0002-2286-1587"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0003-2916-7616"},{"key":"date","value":"2022-04-29"},{"key":"title","value":"SYNGO_MF_4537"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: Figure S2B: Co-purification of BKCa and Cav channels from Xenopus oocytes expressing BKCa (BKα, BKβ4) and Cav2.1 (Cav2.1(I1520H), Cavβ1b or Cavβ3, α2δ) channels.\nFigure 3A: Electrophysiological recordings show that BKCa and Cav2.1 channels functionally are coupled.\nFigure 3B: The coupling between both channels were mandatory for the activation of\nBKCa in the physiological voltage range as shown by control experiments lacking expression of the Cav channels. The amplitude of the Cav channel-activated BKCa current was strongly voltage-dependent and exhibited a bell-shaped steady-state current-voltage (I-V) relation with a peak amplitude at about 20 mV.\n\nIn particular, an increase in open probability and faster activation kinetics of the Cav channels promote accelerated coupling (Fig. 3A) and an increase in the BKCa currents (Fig. 3B), whereas the decrease in Ca2+ current amplitude (due to a reduced driving force at voltages approaching the Ca2+ reversal potential) leads to\nreduction and cessation of BKCa currents at voltages \u003e20 mV (Fig. 3B).\nFigure 6: BKCa currents were eliminated by application of w-agatoxin IVA, a specific blocker of Cav2.1 further confirming the functional coupling between BKCa and Cav2.1.\n\n26/03/2021 Dnyanada: The term \"voltage-gated calcium channel activity..\" was chosen as Cav2.1 (CACNA1A) contributes to an influx of Ca2+ (also represented in Figure 3A). As this Cav2.1 functional couple with the BKCa channel (which are localized in the presynaptic terminal; SynGO ID 1446), it is assumed the effect regulates the presynaptic cytosolic calcium levels.. Experimental description: Electrophysiological recordings from giant inside-out patches excised from oocytes and from CHO cells. The interactors of BKca from the affinity-purification study was conducted in the rat brain.\n\nFrom supplemental M\u0026M;\n\"Preparation and injection of cRNA into Xenopus oocytes and site-directed mutagenesis were done as described (B. Fakler et al., Cell 80, 149 (1995)), cell culturing and transfection of cDNAs were performed as detailed in (J. Ludwig et al., Proc. Natl. Acad. Sci. USA 98, 4178 (2001)). All cDNAs were verified by sequencing; genebank accessions of the clones used are\" ... \"X57477.1 (Cav2.1(I1520H), gift of Dr. J. Yang)\""},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_9986"}]}