{"id":"gomodel:SYNGO_1410","individuals":[{"id":"gomodel:SYNGO_1410266ecd8f-1e80-4aa5-8d3c-43f75266224f","type":[{"type":"class","id":"UniProtKB:O60391","label":"GRIN3B Hsap"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"},{"type":"class","id":"CHEBI:36080","label":"protein"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_141029add384-003d-47a9-af07-28ab845e1724","type":[{"type":"class","id":"ECO:0006063","label":"over expression analysis evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"source","value":"PMID:22564863"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_14106c5c8b0c-1986-4b3a-9506-c867a659aaf0","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"date","value":"2024-03-06"},{"key":"source","value":"PMID:22564863"}]},{"id":"gomodel:SYNGO_141081c6dc3e-19b2-4883-a107-6b1344c05c5e","type":[{"type":"class","id":"ECO:0000303","label":"author statement without traceable support used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"source","value":"PMID:22564863"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_14108b5d5fd6-6941-4e8d-8641-4c6ca1f27485","type":[{"type":"class","id":"ECO:0006013","label":"patch-clamp recording evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"source","value":"PMID:22564863"}]},{"id":"gomodel:SYNGO_1410a0ee626e-37c8-460f-a31e-822a7dea31d9","type":[{"type":"class","id":"GO:0099507","label":"ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]}],"facts":[{"subject":"gomodel:SYNGO_1410a0ee626e-37c8-460f-a31e-822a7dea31d9","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_1410266ecd8f-1e80-4aa5-8d3c-43f75266224f","annotations":[{"key":"evidence","value":"gomodel:SYNGO_141029add384-003d-47a9-af07-28ab845e1724","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_14106c5c8b0c-1986-4b3a-9506-c867a659aaf0","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_141081c6dc3e-19b2-4883-a107-6b1344c05c5e","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_14108b5d5fd6-6941-4e8d-8641-4c6ca1f27485","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2018-06-29"},{"key":"title","value":"SYNGO_MF_1410"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: Expression and electrophysiological measurements of GLUN3B in Xenopus Oocytes.\n\nrule: NAS_MFCC-BP\nrationale:\nProtein has ligand-gated ion channel receptor activity and is localized in the presynaptic membrane (shown with high-resolution technique). The inference to term \u0027ligand-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099507)\u0027 is trivial and non-controversial.\nPinf_CC:\n[Q8VHN2] SyNGO ID 1388 (PMID:23159309, EM). Experimental description: NMDA receptor clones used were GluN1-1a and GluN2A in pRK7, and GluN3A and GluN3B in pcDNA3.1 (gift from Dr. Dongxian Zhang, Burnham Institute, La Jolla, CA), which were confirmed by sequencing. Mutations were introduced using the QuikChange site-directed muta- genesis kit (Stratagene Cloning Systems, UK). Wild-type and mutant plasmids were linearised using the appropriate restriction enzyme and cRNA transcripts were synthesised using the mMESSAGE mMACHINE kit (Ambion).\n\n29/6/2018 Pim\n- I have selected \u0027human\u0027 as the species of the expressed protein. Since using a weaker NAS mechanism for annotation correct species assignment is not so important."},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_9606"}]}