{"id":"gomodel:SYNGO_1331","individuals":[{"id":"gomodel:SYNGO_13310be43f13-9912-4a7d-a955-2f9ffffc25b4","type":[{"type":"class","id":"ECO:0006063","label":"over expression analysis evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:17681556"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"}]},{"id":"gomodel:SYNGO_133117a2429b-d366-4a28-8d1f-a2d2fcdd27bc","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:17681556"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_133124db7d9c-bbb4-4078-bc64-e1fe415614c7","type":[{"type":"class","id":"ECO:0000303","label":"author statement without traceable support used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"source","value":"PMID:17681556"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_1331892cb356-3980-4ca0-9de7-239632fd2d84","type":[{"type":"class","id":"GO:0099507","label":"ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_13319bf72e5f-4e30-4ac5-9ecd-3a67b283254a","type":[{"type":"class","id":"UniProtKB:P31644","label":"GABRA5 Hsap"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"},{"type":"class","id":"CHEBI:36080","label":"protein"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_1331d33cf4e4-ac2f-489e-9320-d83c92098577","type":[{"type":"class","id":"ECO:0006013","label":"patch-clamp recording evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:17681556"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"}]}],"facts":[{"subject":"gomodel:SYNGO_1331892cb356-3980-4ca0-9de7-239632fd2d84","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_13319bf72e5f-4e30-4ac5-9ecd-3a67b283254a","annotations":[{"key":"evidence","value":"gomodel:SYNGO_13310be43f13-9912-4a7d-a955-2f9ffffc25b4","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_133117a2429b-d366-4a28-8d1f-a2d2fcdd27bc","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_133124db7d9c-bbb4-4078-bc64-e1fe415614c7","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_1331d33cf4e4-ac2f-489e-9320-d83c92098577","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2018-06-29"},{"key":"title","value":"SYNGO_MF_1331"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: Electrophysiological characterisation of GABAA alpha 5 subunit overexposed in HEK cells with WC patch clamp.\n\nrule: NAS_MFCC-BP\nrationale:\nProtein has ligand-gated ion channel receptor activity and is localized in the presynaptic membrane (shown with high-resolution technique). The inference to term ligand-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099507) is trivial and non-controversial.\nPinf_CC:\n[P19969] SyNGO ID 1263 (PMID:16998906, EM). Experimental description: The human GABAA receptor a1, a2, a3, a4, a5, a6, b3, and g2L subunits\nwere amplified from the ResGen human brain cDNA library (Invitrogen,\nHuntsville, AL, USA) by polymerase chain reaction (PCR). Full-length\ncDNA clones of a1 and g2L subunits were constructed with the pcDNA3.0 vector (Invitrogen) (Zhao et al., 2006), whilst full-length cDNAs of the other subunits were inserted into the BamHI-XbaI (for a2), KpnI-XbaI (for a6 and b3),\nor BamHI-EcoRI (for a3, a4 and a5) sites of the pcDNA3.1 vector (Invitrogen).\nAll constructs were verified by DNA sequencing."},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_9606"}]}