{"id":"gomodel:SYNGO_1225","individuals":[{"id":"gomodel:SYNGO_12252f44bec9-c8bb-48ae-89de-8d367d624906","type":[{"type":"class","id":"ECO:0006063","label":"over expression analysis evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:12670305"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_122542fd88b2-0e35-45f0-a9b8-4f4f430aa1bd","type":[{"type":"class","id":"ECO:0006013","label":"patch-clamp recording evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:12670305"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_122548778e06-0f61-4b28-ba78-58a2cadcb33c","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"source","value":"PMID:12670305"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_12256dbbe168-c4a3-416d-8e38-0307a9529de4","type":[{"type":"class","id":"GO:0099507","label":"ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_122573c2eb9b-c63b-4140-825c-8b4862ed05c0","type":[{"type":"class","id":"UniProtKB:P48058","label":"GRIA4 Hsap"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"},{"type":"class","id":"CHEBI:36080","label":"protein"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_1225a96b5a9b-c3d3-4c66-953b-8fcbe16e07b8","type":[{"type":"class","id":"ECO:0000303","label":"author statement without traceable support used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"source","value":"PMID:12670305"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"date","value":"2024-03-06"}]}],"facts":[{"subject":"gomodel:SYNGO_12256dbbe168-c4a3-416d-8e38-0307a9529de4","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_122573c2eb9b-c63b-4140-825c-8b4862ed05c0","annotations":[{"key":"evidence","value":"gomodel:SYNGO_12252f44bec9-c8bb-48ae-89de-8d367d624906","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_122542fd88b2-0e35-45f0-a9b8-4f4f430aa1bd","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_122548778e06-0f61-4b28-ba78-58a2cadcb33c","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_1225a96b5a9b-c3d3-4c66-953b-8fcbe16e07b8","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2018-06-29"},{"key":"title","value":"SYNGO_MF_1225"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: Characterisation of AMPA receptor subunits from outside-out patches from transfected HEK cells.\n\nrule: NAS_MFCC-BP\nrationale:\nProtein has ligand-gated ion channel receptor activity and is localized in the presynaptic membrane (shown with high-resolution technique). The inference to term ligand-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099507) is trivial and non-controversial.\nPinf_CC:\n[P19493] SyNGO ID 1204 (PMID:15610164, EM). Experimental description: Human AMPA receptor cDNA was cloned from a human hippocampus cDNAbank(Stratagene,Amsterdam,TheNetherlands).Subunit cDNA was produced by PCR mutagenesis. The constructs were each subcloned into pcDNA3 expression vectors for transfection (Invitro- gen, Groningen, The Netherlands). Transformed human embryonic kidney (HEK) 293 cells were cultured in Dulbecco\u0027s Modified Eagle\u0027s medium (DMEM), supplemented with 10% fetal calf serum, 100 U/ mL penicillin and 100 mg/mL streptomycin at 37 8C in a 5% CO2 : 95% air incubator."},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_9606"}]}