{"id":"gomodel:SYNGO_1209","individuals":[{"id":"gomodel:SYNGO_12091036d884-e1a0-43b8-884f-411a162938bc","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"source","value":"PMID:8824304"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_120935df5871-e2ac-4874-9c56-26bc44b475bf","type":[{"type":"class","id":"ECO:0000303","label":"author statement without traceable support used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:8824304"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_120950d12a7f-2428-45bd-9404-c93b43aea3f6","type":[{"type":"class","id":"UniProtKB:P42261","label":"GRIA1 Hsap"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"},{"type":"class","id":"CHEBI:36080","label":"protein"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_1209592f5195-4637-4bf1-913a-6136735c07c0","type":[{"type":"class","id":"ECO:0006063","label":"over expression analysis evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"source","value":"PMID:8824304"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_12099600148d-d5d7-464e-9fad-e8ee415abb95","type":[{"type":"class","id":"ECO:0006013","label":"patch-clamp recording evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"source","value":"PMID:8824304"}]},{"id":"gomodel:SYNGO_1209f49d9899-1929-4ef6-8e12-9aea36ce31df","type":[{"type":"class","id":"GO:0099507","label":"ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"facts":[{"subject":"gomodel:SYNGO_1209f49d9899-1929-4ef6-8e12-9aea36ce31df","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_120950d12a7f-2428-45bd-9404-c93b43aea3f6","annotations":[{"key":"evidence","value":"gomodel:SYNGO_12091036d884-e1a0-43b8-884f-411a162938bc","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_120935df5871-e2ac-4874-9c56-26bc44b475bf","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_1209592f5195-4637-4bf1-913a-6136735c07c0","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_12099600148d-d5d7-464e-9fad-e8ee415abb95","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2018-06-29"},{"key":"title","value":"SYNGO_MF_1209"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: electrophysiological characterisation of GluR1 subunit in HEK cells using WC patch clamp.\n\nrule: NAS_MFCC-BP\nrationale:\nProtein has ligand-gated ion channel receptor activity and is localized in the presynaptic membrane (shown with high-resolution technique). The inference to term ligand-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099507) is trivial and non-controversial.\nPinf_CC:\n[P19490] SyNGO ID 1203 (PMID:15610164, EM). Experimental description: Plasmids and mutagenesis. cDNAs for wild-type GluRAi (flip) and GluRAo (flop) in CMV expression vectors were generous gifts of Dr. Peter Seeburg (Heidelberg, Germany). Point mutations were made in pBS/GluRAi by dut-ung- oligonucleotide missense mutagenesis (Bio-Rad Muta-Gene Phagemid In Vitro Mutagenesis Kit, Version 2; Bio-Rad, Hercules, CA) and then shuttled into the CMV expression clone using the BspE13BspE1 (New England Biolabs, Beverly, MA) fragment of GluRA (nt 1777-2320), after which the sequence of the mutation was confirmed. S750N, S750Q, GluRAi[io], and GluRAo[oi] were described previously (Partin et al., 1995). All cDNAs were purified through two cesium chloride gradients before transfection.\n\n29/6/2018 Pim\n- I have selected \u0027human\u0027 as the species of the expressed protein. Since using a weaker NAS mechanism for annotation correct species assignment is not so important."},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_9606"}]}