{"id":"gomodel:SYNGO_1191","individuals":[{"id":"gomodel:SYNGO_119106d7d8f1-c7c5-4742-932e-428871a306cf","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"source","value":"PMID:8137830"}]},{"id":"gomodel:SYNGO_11910846b12c-6c8a-4c99-9db5-73403ce4b2bf","type":[{"type":"class","id":"ECO:0000303","label":"author statement without traceable support used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:8137830"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"date","value":"2024-03-06"}]},{"id":"gomodel:SYNGO_119109d06052-cccc-4a1b-b0aa-c99edf15591f","type":[{"type":"class","id":"UniProtKB:P23415","label":"GLRA1 Hsap"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"},{"type":"class","id":"CHEBI:36080","label":"protein"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_11913192adc9-49dc-48bd-9656-1f8dbcd63551","type":[{"type":"class","id":"GO:0099507","label":"ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_119139b88cdc-f2b4-42ed-8b7b-efeaca28eea5","type":[{"type":"class","id":"ECO:0006063","label":"over expression analysis evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"source","value":"PMID:8137830"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_1191bb5385dd-ce40-4aca-80dd-96bffb662e17","type":[{"type":"class","id":"ECO:0006013","label":"patch-clamp recording evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"source","value":"PMID:8137830"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"}]}],"facts":[{"subject":"gomodel:SYNGO_11913192adc9-49dc-48bd-9656-1f8dbcd63551","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_119109d06052-cccc-4a1b-b0aa-c99edf15591f","annotations":[{"key":"evidence","value":"gomodel:SYNGO_119106d7d8f1-c7c5-4742-932e-428871a306cf","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_11910846b12c-6c8a-4c99-9db5-73403ce4b2bf","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_119139b88cdc-f2b4-42ed-8b7b-efeaca28eea5","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_1191bb5385dd-ce40-4aca-80dd-96bffb662e17","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2018-06-29"},{"key":"title","value":"SYNGO_MF_1191"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: Expression of Glycine receptor subunits to show functional (ion-channel) properties, here measured by single channel measurements in out-side-out patch configuration.\n\nrule: NAS_MFCC-BP\nrationale:\nProtein has ligand-gated ion channel receptor activity and is localized in the presynaptic membrane (shown with high-resolution technique). The inference to term ligand-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099507) is trivial and non-controversial.\nPinf_CC:\n[P07727] SyNGO ID 1129 (PMID:23175852, EM). Experimental description: For transfection, the human al and cx2 (Grenningloh, 1990a), ratc3 (Kuhse etal.,1990)andratfl(Grenninglohetal.,1990b)subunitcDNAs were insertedintothemammalianexpressionvectorspCIS2(Gormanetal.,1989) orpRC/CMV (Invitrogen),bothofwhichcarryacytomegaloviruspromoter.\n\np.3730: Expression of homoand hetero-oligomeric GlyRsTransfectionofHEK-293cellswitha 1, U2orO3subunitcDNAs resulted in the formation of functional homo-oligomericchannels,whichwere studiedbywhole-cellrecording. -- Highlighted 07 Aug 2017"},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_9606"}]}