{"id":"gomodel:SYNGO_1135","individuals":[{"id":"gomodel:SYNGO_11353b290659-07ba-4749-85d6-9780834143d6","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"date","value":"2024-03-06"},{"key":"source","value":"PMID:25989383"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_113583168c6c-8941-492e-94d1-6b648d41f827","type":[{"type":"class","id":"ECO:0006063","label":"over expression analysis evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"source","value":"PMID:25989383"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"providedBy","value":"https://syngo.vu.nl"}]},{"id":"gomodel:SYNGO_11358e0c40eb-6916-47cc-a82c-547385c8f862","type":[{"type":"class","id":"ECO:0000303","label":"author statement without traceable support used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:25989383"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_1135bc66bb6c-3505-4424-9b43-dfb77974e352","type":[{"type":"class","id":"GO:0099507","label":"ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_1135bdf504f0-7b02-4f1a-9845-69ce8f243f41","type":[{"type":"class","id":"MGI:MGI:96282","label":"Htr3a Mmus"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"}]},{"id":"gomodel:SYNGO_1135f8d89441-870b-4e97-b33b-cba4e0f00107","type":[{"type":"class","id":"ECO:0006013","label":"patch-clamp recording evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"date","value":"2024-03-06"},{"key":"source","value":"PMID:25989383"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]}],"facts":[{"subject":"gomodel:SYNGO_1135bc66bb6c-3505-4424-9b43-dfb77974e352","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_1135bdf504f0-7b02-4f1a-9845-69ce8f243f41","annotations":[{"key":"evidence","value":"gomodel:SYNGO_11353b290659-07ba-4749-85d6-9780834143d6","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_113583168c6c-8941-492e-94d1-6b648d41f827","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_11358e0c40eb-6916-47cc-a82c-547385c8f862","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_1135f8d89441-870b-4e97-b33b-cba4e0f00107","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2021-02-17"},{"key":"title","value":"SYNGO_MF_1135"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: 5-HT3A receptro subunit characterisation in HEK cells.\n\nrule: NAS_MFCC-BP\nrationale:\nProtein has ligand-gated ion channel receptor activity and is localized in the presynaptic membrane (shown with high-resolution technique). The inference to term ligand-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099507) is trivial and non-controversial.\nPinf_CC:\n[P35563] SyNGO ID 1133 (PMID:11876772, EM). Experimental description: The mouse 5-HT3A subunit was tagged with a linker sequence and two bungarotoxin pharmatope-tags on the receptor C-terminus (5-HT3A/BBS) in pCDNA3.1. The human 5-HT3A subunit was tagged with the hemagglutinin (HA) epitope in pCDNA3.1 vector on the N-terminus. Mouse 5-HT3A/BBS and human 5-HT3A subunits were stably expressed in HEK-293 cells by transfection with polyethyl- eneimine (Sigma-Aldrich, St. Louis, MO, USA) (150 μM per 1 μg DNA) or lipofectamine (Invitrogen) respectively. Cells were incubated for 24-48 h at 37°C in 5% CO2 after transfec- tion to allow for expression, yielding HEK-3A/BBS or HEK-h3A cells respectively."},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_10090"}]}