{"id":"gomodel:SYNGO_1117","individuals":[{"id":"gomodel:SYNGO_111731b8b4e1-4e63-4e85-ab6b-f32b58edeb40","type":[{"type":"class","id":"GO:0099507","label":"ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"}],"root-type":[{"type":"class","id":"GO:0003674","label":"molecular_function"},{"type":"class","id":"obo:go/extensions/reacto.owl#molecular_event","label":"Molecular Event"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"}]},{"id":"gomodel:SYNGO_11174705a6e1-87a5-4404-91be-e4b151ac5cb1","type":[{"type":"class","id":"ECO:0006013","label":"patch-clamp recording evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:1373382"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_111750c36bcc-125f-4284-b8c7-35036a2b6d40","type":[{"type":"class","id":"ECO:0006063","label":"over expression analysis evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"source","value":"PMID:1373382"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"}]},{"id":"gomodel:SYNGO_11176458e7c8-c35b-4c5d-af14-ba0afc684ac8","type":[{"type":"class","id":"RGD:2732","label":"Grik1 Rnor"}],"root-type":[{"type":"class","id":"CHEBI:33695","label":"information biomacromolecule"},{"type":"class","id":"CHEBI:24431","label":"chemical entity"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_1117ac14576f-80e6-4f26-9f57-86af93944523","type":[{"type":"class","id":"ECO:0007695","label":"cell-based assay evidence used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"source","value":"PMID:1373382"},{"key":"date","value":"2024-03-06"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"}]},{"id":"gomodel:SYNGO_1117c6fecf53-a54d-4908-94c2-996108426aa6","type":[{"type":"class","id":"ECO:0000303","label":"author statement without traceable support used in manual assertion"}],"root-type":[{"type":"class","id":"ECO:0000000","label":"evidence"}],"annotations":[{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"source","value":"PMID:1373382"}]}],"facts":[{"subject":"gomodel:SYNGO_111731b8b4e1-4e63-4e85-ab6b-f32b58edeb40","property":"RO:0002333","property-label":"RO:0002333","object":"gomodel:SYNGO_11176458e7c8-c35b-4c5d-af14-ba0afc684ac8","annotations":[{"key":"evidence","value":"gomodel:SYNGO_11174705a6e1-87a5-4404-91be-e4b151ac5cb1","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_111750c36bcc-125f-4284-b8c7-35036a2b6d40","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_1117ac14576f-80e6-4f26-9f57-86af93944523","value-type":"IRI"},{"key":"evidence","value":"gomodel:SYNGO_1117c6fecf53-a54d-4908-94c2-996108426aa6","value-type":"IRI"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2024-03-06"},{"key":"providedBy","value":"https://syngo.vu.nl"}]}],"annotations":[{"key":"state","value":"production"},{"key":"contributor","value":"https://orcid.org/0000-0001-9425-2935"},{"key":"contributor","value":"https://orcid.org/0000-0002-2514-0216"},{"key":"date","value":"2018-06-29"},{"key":"title","value":"SYNGO_MF_1117"},{"key":"providedBy","value":"https://syngo.vu.nl"},{"key":"comment","value":"Rationale: Kainate receptor GluR5 (\u003dGRIK1).\n\nrule: NAS_MFCC-BP\nrationale:\nProtein has ligand-gated ion channel receptor activity and is localized in the presynaptic membrane (shown with high-resolution technique). The inference to term ligand-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099507) is trivial and non-controversial.\nPinf_CC:\n[P22756] SyNGO ID 1114 (PMID:16360275, EM). Experimental description: Isolation and expression of GluR-5 clones\nForPCR (MullisandFalooona, 1987),oligonucleotideprimersandreaction conditionswereasdescribed(Keinanenetal.,1990).RatforebraincDNA was used as a template. The PCR product (- 500 bp) was gel-purified, cleaved with EcoRI and KpnI, subcloned into doubly cut M13mpl9 RF- DNA (Yanisch-Perronetal.,1985)andsequenced(Sangeretal.,1977). A PCR-generatedDNA fragmentofaGluR-5specificM 13subclonewas internalylabelledwith[3P]dCTPtoaspecificactivityof2x 109/Igand usedasaprobetoscreenaratforebraincDNA libraryconstructedinX- Zap (Stratagene). Five independent cDNAs from these screens were subclonedintopBluescriptSK plasmidvectors(Stratagene,CA) orM13mpl8 andmpl9RF-DNA (Yanisch-Perronetal.,1985)andthesesubcloneswere used for sequence analysis (Sanger et al., 1977). Expression constructs of GluR-5-2 specifying different C-termini were engineered by subcloning 3\u0027 BamnHI-AoI or BamHI-EcoRI fragments encoding C-termini of GluR-5-2aor-2-c,respectively,anda5\u0027HindLH-BamHI fragmentdirectly intoamammalianexpressionvectorwithaCMV promoter(Gormanetal.,\n1990).TheheterogeneityconcerningglutamineandargininecodonsinTM2 wasgeneratedbyexchanginga400bpBspEI-MscIfragmentcoveringTM regionsItoImofeditedandnon-editedGIuR-5cDNA clones. Theconstructs wereusedtotransfecthumanembryonickidneycels293(ATCC CRL 1573)asdescribed(ChenandOkayama, 1987;Pritcheletal.,1989).These were subjected to binding studies and electrophysiology."},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_10116"}]}