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SynArfGEF/BRAG3/IQSEC3 localizes to postsynaptic specializations of symmetrical, inhibitory synapses. Immunohistochemical analysis of the mouse forebrain, cerebellum, and spinal cord revealed that IQSEC3 colocalizes with gephrin, a component of inhibitory postsynaptic specializations (Fig. 1e-g, o-q; s-u; Fig.4b-d, k-n), but not with PSD-95, a component of the postsynaptic density of asymmetric, excitatory synapses (Fig.1h-i). Further, IQSEC3 immunoreactivity colocalizes with the staining pattern of GABAARs (Fig.4e-g) and GlyRs (Fig.4k-n), but not of GluA2Rs (Fig.h-j). Pre-embedding immuno-EM confirmed the presence of IQSEC3-immunoreactivity at postsynaptic specializations of symmetrical synapses (Fig.5).\n\nAntibodies (rabbit and guinea pig) were generated in this study using a GST-IQSEC3 fusion protein and specificity was tested by Western Blot (Fig.2) and for IHC by pre-absorption of the antigen (Fig.3b).. Experimental description: Under deep anesthesia with diethyl ether, C57BL/6N male mice at postnatal 10-12 weeks transcardially fixed with 4% paraformaldehyde"},{"key":"https://w3id.org/biolink/vocab/in_taxon","value":"http://purl.obolibrary.org/obo/NCBITaxon_10090"}]}